A New High Performance Liquid Chromatographic Technique for Separation and Determination of Adenylic and Nicotinamide Nucleotides in Lactobacillus Plantarum
نویسندگان
چکیده
A new technique for separation and dctermination of ATP, ADP, AMP, NAD', NADP', NADH and NADPH in Lactobacillus plantarum has been developped. It involves acid and basic extraction of nucleotide pool, analysis by reverse-phase high performance liquid chromatography on a 5 p Spherisorb ODS-1 column and UV detection. The method offers advantages in accurate estimations of adenylic and nicotinamide nucleotides concentrations in Lactobacillus plantarunt during the growth phase. Such results indicate the potential of this technique as an important research tool.
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